Effects of seasonality and developed land cover on Culex mosquito abundance and microbiome diversity.

The vectorial capacity of mosquitoes, which influences the dynamics of vector-borne disease transmission, is intricately linked to mosquito abundance and the composition and diversity of their associated microbiomes. However, the influence of environmental factors on mosquito populations and microbiome diversity remains underexplored. Here we examined the effects of seasonality and developed land cover on Culex mosquito abundance and bacterial diversity. Biweekly field sampling of female Culex mosquitoes was conducted using gravid and CDC light traps, spanning summer to autumn across varying developed land cover levels in two urban areas in Central Illinois. Mosquito abundance was assessed by the number of mosquitoes captured per trap night and compared across seasons and developed levels. The mean mosquito abundance for gravid and light traps was 12.96 ± 2.15 and 7.67 ± 1.44, respectively. Notably, higher levels of developed land cover exhibited higher Culex abundance than the low level for light traps, but no significant difference was found between summer and early autumn. In gravid traps, no significant differences were detected across seasons or developed levels. Microbial analysis of the mosquito microbiome revealed that Proteobacteria and Wolbachia, with a mean relative abundance of 80.77 and 52.66% respectively, were identified as the most dominant bacterial phylum and genus. Their relative abundance remained consistent across seasons and developed land cover levels, with negligible variations. Alpha diversity, as measured by observed species, Chao1, Shannon, and Simpson, showed slightly higher values in early-autumn compared to late-summer. A notable pattern of bacterial diversity, as indicated by all four diversity indexes, is evident across varying levels of land development. Significantly, high or intermediate developed levels consistently showed reduced alpha diversity when compared to the lower level. This underscores the pronounced impact of anthropogenic ecological disturbances in shaping mosquito microbiomes. Beta diversity analysis revealed no significant dissimilarities in bacterial community composition across seasons and developed levels, although some separation was noted among different levels of developed land cover. These findings highlight the significant role of environmental factors in shaping mosquito abundance and their associated microbiomes, with potential implications for the vectorial capacity in the transmission of vector-borne diseases.

Stability near threshold in a semiconductor laser subject to optical feedback: a bifurcation analysis of the Lang-Kobayashi equations.

Through the use of analytical and numerical techniques, we investigate the interaction between the trivial off-state and the continuous-wave (CW) operation of a semiconductor laser subject to conventional optical feedback. More specifically, using numerical continuation tools, the stability and bifurcations of the CW states, or external-cavity modes (ECMs), are analyzed in dependence on the parameters of feedback phase, feedback strength, pump current, and the linewidth enhancement factor. In this way, curves of codimension-one Hopf bifurcations are shown to destabilize the off-state and lead to stable ECM operation. Moreover, self-intersections of these Hopf curves in codimension-two Hopf-Hopf bifurcation points are seen to give rise to curves of codimension-one torus bifurcations (Hopf bifurcations of the ECMs), and degenerate-Hopf points to the birth of saddle-node bifurcations of the ECMs, as parameters are varied. These codimension-two points are shown to come together at a codimension-three degenerate Hopf-Hopf point (a Bogdanov-Takens bifurcation of the ECMs): a limiting point for which a stable off-state can exist.

Analysis of effect of casein phosphopeptides on zinc binding using mass spectrometry.

Phosphorylation is one of the key events in signal transduction and zinc plays an important catalytic and/or structural role in many biological systems. The binding of Zn to a phosphopeptide will alter the physiological functions of a peptide. The binding of casein phosphopeptides (CPPs) to Zn has been analyzed using nanospray mass spectrometry. Electrospray ionization (ESI) spectra of peptides produced by tryptic digestion of alpha-casein incubated with Zn show both free and Zn-bound phosphopeptides. The interaction of CPPs and the corresponding dephosphorylated peptides with zinc is compared. This study demonstrates that the phosphorylation state of a peptide dramatically affects Zn binding, with the decrease in Zn-bound forms of peptide paralleling the decrease in phosphorylation as casein is chemically dephosphorylated, although, in some cases, a small amount of residual Zn-binding capacity remains in the completely dephosphorylated peptide. The observed fragmentation patterns of the Zn-bound CPPs support the thesis that nonphosphorylated residues are involved in the metal binding.

Numerical stability analysis of a large-scale delay system modeling a lateral semiconductor laser subject to optical feedback.

This paper highlights the use of advanced numerical tools to study the stability of large-scale systems of delay differential equations (DDEs). Specifically, we consider a model describing a semiconductor laser subject to conventional optical feedback and lateral carrier diffusion. The symmetry of the governing rate equations allows external cavity mode solutions (ECMs) to be computed as steady state solutions. Using the software package DDE-BIFTOOL, branches of ECMs are computed as a function of varying feedback strength. The stability along these branches is computed by solving eigenvalue problems, the size of which is governed by a step-length heuristic. In this paper, we employ an improved heuristic which substantially reduces the size of these eigenvalue problems. This approach makes the stability analysis of large-scale systems of DDEs computationally feasible.

Enzyme-assisted suicide: molecular basis for the antifungal activity of 5-hydroxy-4-oxonorvaline by potent inhibition of homoserine dehydrogenase.

The structure of the antifungal drug 5-hydroxy-4-oxonorvaline (HON) in complex with its target homoserine dehydrogenase (HSD) has been determined by X-ray diffraction to 2.6 A resolution. HON shows potent in vitro and in vivo activity against various fungal pathogens despite its weak (2 mM) affinity for HSD in the steady state. The structure together with structure-activity relationship studies, mass spectrometry experiments, and spectroscopic data reveals that the molecular mechanism of antifungal action conferred by HON involves enzyme-dependent formation of a covalent adduct between C4 of the nicotinamide ring of NAD(+) and C5 of HON. Furthermore, novel interactions are involved in stabilizing the (HON*NAD)-adduct, which are not observed in the enzyme's ternary complex structure. These findings clarify the apparent paradox of the potent antifungal actions of HON given its weak steady-state inhibition characteristics.

External cavity modes of semiconductor lasers with phase-conjugate feedback.

External cavity modes (ECMs) of a semiconductor laser with phase-conjugate feedback are defined as time-periodic pulsating intensity solutions exhibiting a frequency close to an integer multiple of the external cavity frequency. As the feedback rate progressively increases from zero, they sequentially appear as stable attractors in the bifurcation diagram. We construct a simple analytical approximation of these pulsating intensity solutions and determine their frequencies. We show that branches of ECMs are isolated. Finally, the validity of our approximation is tested by comparing numerical bifurcation diagrams obtained by simulation and continuation techniques with our analytical results.

Global bifurcations and bistability at the locking boundaries of a semiconductor laser with phase-conjugate feedback.

We investigate dynamics and bifurcations of a single-mode semiconductor laser subject to phase-conjugate feedback near the locking region. The system is described by rate equations which are a three-dimensional system with a delay. With tools that go much beyond mere simulation, we find and follow steady states regardless of their stability and compute unstable manifolds of saddle points. Furthermore, we identify heteroclinic bifurcations, which turn out to be responsible for bistability and excitability at the locking boundaries.